ABSTRACT
OBJECTIVE@#To explore the feasibility of improving the sensitivity of DNA detection by increasing the PCR cycle index and decreasing the volume of amplifying system.@*METHODS@#The DNA of semen were collected from 10 healthy irrelevant volunteers, and were quantified to 50, 40, 30, 25, 20, 15, 10 pg/microL, separately. All samples were then amplified in 10, 5, 3 microL volume and at 28, 30, 32, 34, 36 cycles, respectively. 3130 genetic analyzer was used to detect 15 autosomal STR loci.@*RESULTS@#Under the situation of 28 cycles and 3 microL volume, samples which achieved > 40 pg/microL could be correctly typed. Under the situation of 10, 5, 3 microL volume, samples which achieved > 20 pg/microL could be correctly typed at 34 cycles. When increasing the index to 36 cycles, they could not be correctly typed because of the non-specific band.@*CONCLUSION@#DNA detecting sensitivity can be improved to a certain extent by increasing the cycle index and decreasing the volume of amplifying system.
Subject(s)
Humans , Male , DNA/genetics , DNA Fingerprinting/methods , Feasibility Studies , Forensic Genetics/methods , Limit of Detection , Polymerase Chain Reaction/methods , Semen/chemistry , Sensitivity and Specificity , Tandem Repeat SequencesABSTRACT
OBJECTIVE@#To investigate the single nucleotide polymorphisms (SNP) of -855 G/C and -1140 G/A in promoter regions of GRIN1 gene and find their genetic correlation to paranoid schizophrenia as well as their applicable values in forensic medicine.@*METHODS@#The genetic polymorphisms of -855 G/C and -1140 G/A at the 5' end of GRIN1 gene were detected by PCR restriction fragment length polymorphism and PAGE in 183 healthy unrelated individuals of northern Chinese Han population and 172 patients of paranoid schizophrenia, respectively. The chi2 test was used to identify Hardy-Weinberg equilibrium of the genotype distribution. The differences of genotypes and allelic frequency distributions were compared between the two groups.@*RESULTS@#Distributions of the genotypic frequencies satisfied Hardy-Weinberg equilibrium in both groups. The difference of genotypes was statistically significant between female patient group and female control group in -855 G/C distribution (P < 0.05). The differences of genotypes and allelic frequencies were statistically significant not only between the patient group and the control group but also between female patient group and female control group in -1140 G/A distribution (P < 0.05).@*CONCLUSION@#The SNP of -1140 G/A in promoter regions of GRIN1 gene might positively correlate to paranoid schizophrenia. The genetic factor of schizophrenia is involved in gender tendency. And it could be useful in forensic identification of schizophrenia.
Subject(s)
Female , Humans , Male , Alleles , Asian People/genetics , Base Sequence , Gene Frequency , Genetic Predisposition to Disease/genetics , Genotype , Nerve Tissue Proteins/genetics , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Polymorphism, Single Nucleotide , Promoter Regions, Genetic , Receptors, N-Methyl-D-Aspartate/genetics , Schizophrenia, Paranoid/genetics , Sequence Analysis, DNAABSTRACT
OBJECTIVE@#To reveal the genetic polymorphisms of four SNP loci (rs77434921, rs2076907, rs6283, rs1800762) in D5 gene of dopamine receptor (DRD5) in Northern Chinese Han population.@*METHODS@#Four SNP loci of the DRD5 gene of 206 unrelated individuals in Northern Chinese Han population were separately amplified and sequenced by PCR technique and statistically analyzed by Haploview v4.1 software.@*RESULTS@#In Northern Chinese Han population, the genotype frequency distribution of rs77434921, rs2076907, rs6283 and rs1800762 loci in the DRD5 gene were all in accordance with Hardy-Weinberg equilibrium. DP value was 0.145, 0.532, 0.602 and 0.159, while PE value was 0.004, 0.079, 0.196 and 0.007. A linkage disequilibrium among these four SNP loci was also demonstrated, which might infer five haplotypes.@*CONCLUSION@#rs2076907 and rs6283 loci of DRD5 gene in the Northern Chinese Han population have high genetic polymorphisms, which can be useful for forensic identification of individuals.
Subject(s)
Humans , Asian People/genetics , China/ethnology , DNA Primers/genetics , Forensic Genetics , Gene Frequency , Genetic Markers , Genotype , Haplotypes , Linkage Disequilibrium , Polymerase Chain Reaction , Polymorphism, Single Nucleotide , Receptors, Dopamine D5/geneticsABSTRACT
OBJECTIVE@#To investigate the genetic polymorphism in the 5' and 3' region of TPH2 gene of Northern Chinese Han population and to explore its application value in forensic medicine.@*METHODS@#The sequence variants and the genetic polymorphisms of 6 SNP loci (rs4570625, rs11178997, rs11178998, rs41317118, rs17110747 and rs41317114) within a 905 bp 5' flanking region and a 1,104bp 3' flanking region of TPH2 gene were analyzed by DNA sequencing in a total of 244 unrelated healthy individuals in Northern Chinese Han population. The statistical analysis was carried out by Haploview v4.2 software.@*RESULTS@#The genotypic distributions of the 6 SNP loci in the TPH2 gene were in accordance with Hardy-Weinberg equilibrium. One C/T variant in 92922 site was found. There was a high linkage disequilibrium among the 3 SNP loci (rs4570625, rs11178997 and rs11178998) in the 5' region and the 3 SNP loci (rs41317118, rs17110747 and rs41317114) in the 3' region of TPH2 gene, respectively. The parameters of population genetics of 6 SNP loci were obtained.@*CONCLUSION@#There are great polymorphisms in the 5' and 3' region of TPH2 gene in Northern Chinese Han population, which could be used as genetic indexes for association analysis of the related diseases, as well as for forensic individual identification and paternity testing.
Subject(s)
Humans , 3' Untranslated Regions , 5' Untranslated Regions , Asian People/genetics , China/ethnology , Forensic Genetics , Gene Frequency , Genetics, Population , Genotype , Linkage Disequilibrium/genetics , Microsatellite Repeats , Polymerase Chain Reaction , Polymorphism, Single Nucleotide , Tryptophan Hydroxylase/geneticsABSTRACT
OBJECTIVE@#To investigate the population genetic data of 3 SNP loci (rs25533, rs34388196 and rs1042173) of 5-hydroxytryptamine transporter (5-HTT) gene and the association with paranoid schizophrenia.@*METHODS@#Three SNP loci of 5-HTT gene were examined in 132 paranoid schizophrenia patients and 150 unrelated healthy individuals of Northern Chinese Han population by PCR-RFLP technique. The Hardy-Weinberg equilibrium test was performed using the chi-square test and the data of haplotype frequency and population genetics parameters were statistically analyzed.@*RESULTS@#Among these three SNP loci, four haplotypes were obtained. There were no statistically significant differences between the patient group and the control group (P > 0.05). The DP values of the 3 SNP loci were 0.276, 0.502 and 0.502. The PIC of them were 0.151, 0.281 and 0.281. The PE of them were 0.014, 0.072 and 0.072.@*CONCLUSION@#The three SNP loci and four haplotypes of 5-HTT gene have no association with paranoid schizophrenia, while the polymorphism still have high potential application in forensic practice.
Subject(s)
Adult , Female , Humans , Male , Asian People/genetics , Case-Control Studies , Gene Frequency , Genetic Predisposition to Disease , Genotype , Haplotypes , Polymerase Chain Reaction/methods , Polymorphism, Single Nucleotide/genetics , Schizophrenia, Paranoid/genetics , Serotonin Plasma Membrane Transport Proteins/geneticsABSTRACT
OBJECTIVE@#To investigate the polymorphisms of rs4906902 and rs8179184 loci in the promoter of the gamma-aminobutyric acid(GABA) receptor A, beta3 subunit gene (GABRB3), and their relevance with schizophrenia.@*METHODS@#PCR and DNA sequencing were used to detect the polymorphisms of rs4906902 and rs8179184 loci in 210 healthy individuals (control group) and 206 schizophrenic patients (case group) of the Han population in northern China. The chi2 test was used to identify Hardy-Weinberg equilibrium of the genotype distribution in the control group followed by comparing differences in genotype and haplotype frequency distributions between two groups.@*RESULTS@#Distributions of the genotype frequencies fit the law of Hardy-Weinberg equilibrium in the control group. rs4906902 and rs8179184 loci were in linkage disequilibrium and showed two haplotypes which were T-G and C-A. The differences of genotypic frequencies and haplotype frequencies were statistically significant between the two groups (P < 0.05). The frequency of haplotype C-A in the case group was significantly higher than in the control group. Genotypic and haplotype frequencies in the maternal line and paternal line were statistically significant in the case group (P < 0.05).@*CONCLUSION@#The haplotype of C-A in rs4906902 and rs8179184 loci in the promoter of GABRB3 gene may be maternally inherited and positively associated with schizophrenia and may be a useful tool in the forensic identification of schizophrenia.
Subject(s)
Female , Humans , Male , Alleles , Asian People/genetics , China/epidemiology , Gene Frequency , Genetic Predisposition to Disease , Genotype , Haplotypes , Inheritance Patterns , Linkage Disequilibrium , Polymerase Chain Reaction , Polymorphism, Single Nucleotide , Promoter Regions, Genetic , Receptors, GABA-A/genetics , Schizophrenia/genetics , Sequence Analysis, DNAABSTRACT
OBJECTIVE@#To investigate the polymorphism of cholecystokinin (CCK) gene -45C/T of schizophrenia and its application in forensic medicine.@*METHODS@#Bidirectional allele specific PCR was used to detect CCK gene -45C/T polymorphisms in 207 schizophrenic patients (case group) and 202 healthy individuals (control group) of the Han population in northern China. The chi2 test was used to identify Hardy-Weinberg equilibrium of the genotype distribution in control group. The differences of genotype and allele frequencies distributions were compared between two groups.@*RESULTS@#Distributions of the genotype frequencies satisfied the law of Hardy-Weinberg equilibrium in control group. The differences between genotypic frequencies and allele frequencies were not statistical significance in case group and control groups (P > 0.05). Gender-stratified analysis showed that frequency of allele T in female case group was statistically higher than that in female control group (P = 0.044).@*CONCLUSION@#CCK gene -45C/T locus T allele may be positively associated with schizophrenia in female population and useful in schizophrenia identification.
Subject(s)
Female , Humans , Male , Alleles , Asian People/genetics , Case-Control Studies , China , Cholecystokinin/genetics , Forensic Genetics , Forensic Psychiatry , Gene Frequency , Genetic Predisposition to Disease , Genotype , Polymerase Chain Reaction , Polymorphism, Genetic , Schizophrenia/genetics , Sequence Analysis, DNAABSTRACT
OBJECTIVE@#To investigate the sequence features of FUT2/01 locus and its polymorphic distribution in Chinese population, and to discuss its application potential in forensic medicine.@*METHODS@#The alleles on FUT2/01 locus were amplified by PCR and then were sequenced. Furthermore, polymorphic distribution of the locus was analyzed by polyacrylamide gel electrophoresis. The genotypes were characterized with fluorescence labeling followed by automatic detection system.@*RESULTS@#The sequencing results only showed the length differences which were determined by the tandem repeats variance of the core sequence. There were 9 alleles and 28 genotypes identified from 162 individuals. The discrimination power and excluding probability of paternity were 0.9639 and 0.6266, respectively. In addition, the locus could be genotyped by automatic analysis very well.@*CONCLUSION@#The FUT2/01 locus exhibits high heterozygosity and individual identification power in Chinese Han population, and may be a valuable STR system for application in forensic medicine.
Subject(s)
Humans , Alleles , Asian People , Base Sequence , China/ethnology , Electrophoresis, Polyacrylamide Gel , Fucosyltransferases/genetics , Gene Frequency , Genetics, Population , Genotype , Polymerase Chain Reaction/methods , Polymorphism, Genetic , Sequence Analysis, DNA , Tandem Repeat Sequences/geneticsABSTRACT
OBJECTIVE@#To study the distribution of the tryptophan hydroxylase (TPH) gene-T457C locus polymorphism in Han ethnic group in northern China and to find its applicable value in forensic science.@*METHODS@#Genomic DNA samples, extracted from 180 unrelated individuals in northern Chinese Han population, were analyzed by PCR-RFLP.@*RESULTS@#The discrimination power (DP) value and the power of exclusion (PE) value of the TPH gene-T457C locus were 0.624 and 0.187, respectively. The allele frequency showed significant difference from that of French people (P=0.04).@*CONCLUSION@#Polymorphism of the TPH gene-T457C locus could show ethnic and regional differences. It has a potential to be used in forensic science.